Anti-Human NELFE Polyclonal Antibody (HB121014)

Name: Anti-Human NELFE Polyclonal Antibody
Brand: abinScience
SKU: HB121014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB121014

Описание

Anti-Human NELFE Polyclonal Antibody (HB121014) is a rabbit polyclonal antibody detecting NELFE in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NELFE (Gly261-Arg331).

Цель

RDBP, RD, NELF-E, RNA-binding protein RD, NELFE, Negative elongation factor E

Очистка

Purified by antigen affinity column.

Номер доступа

P18615

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Negative elongation factor E (NELFE) is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.

1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401

2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882

3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650

4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062

5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858

6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human NELFE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа