Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014)

Name: Anti-Human NEU3/Sialidase-3 Polyclonal Antibody
Brand: abinScience
SKU: HP187014
Price: 96 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HP187014

Description

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014) is a rabbit polyclonal antibody detecting Sialidase-3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NEU3/Sialidase-3 (Met1-Asn428).

Cible

N-acetyl-alpha-neuraminidase 3, NEU3, Sialidase-3, Ganglioside sialidasedis, Membrane sialidase

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9UQ49

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Sialidase-3 (NEU3) is a ~48 kDa protein. Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.

1. Wada, T. et al. (1999) Biochemical and biophysical research communications 261, 21-7. PMID: 10405317

2. Monti, E. et al. (2000) The Biochemical journal 349, 343-51. PMID: 10861246

3. Wang, Y. et al. (2001) European journal of biochemistry 268, 2201-8. PMID: 11298736

4. Wang, Y. et al. (2002) The Journal of biological chemistry 277, 26252-9. PMID: 12011038

5. Yamaguchi, K. et al. (2005) The Biochemical journal 390, 85-93. PMID: 15847605

6. Albohy, A. et al. (2010) Glycobiology 20, 1127-38. PMID: 20511247

7. Rodriguez-Walker, M. et al. (2017) Scientific reports 7, 4167. PMID: 28646141

8. Wada, T. et al. (2007) Oncogene 26, 2483-90. PMID: 17334392

9. Mozzi, A. et al. (2015) Glycobiology 25, 855-68. PMID: 25922362

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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Kits d'Essai

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014)

Aperçu

Images

Reférences

Fiche technique

SDS