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Anti-Human NKX2-5 Polyclonal Antibody (HW502014)

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Aperçu
Numéro de catalogueHW502014
Description
Anti-Human NKX2-5 Polyclonal Antibody (HW502014) is a rabbit polyclonal antibody detecting NKX2-5 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human NKX2-5 (Ser146-Gly206).
Cible NKX2E, CSX, Homeobox protein Nkx-2.5, NKX2.5, NKX2-5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E
Purification Purified by antigen affinity column.
Numéro d'accès P52952
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Homeobox protein Nkx-2.5 (NKX2-5) is a ~34 kDa protein. Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4. May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal. Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression.

1. Koss, M. et al. (2012) Developmental cell 22, 913-26. PMID: 22560297
2. Pradhan, L. et al. (2012) Biochemistry 51, 6312-9. PMID: 22849347
3. Pradhan, L. et al. (2016) Biochemistry 55, 1702-10. PMID: 26926761
4. Samokhin, AO. et al. (2018) Science translational medicine 10. PMID: 29899023
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human NKX2-5 Polyclonal Antibody

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