Anti-Human Notch-2/Notch-3 Recombinant Antibody (SAA0143) (HC513107)

Name: Anti-Human Notch-2/Notch-3 Recombinant Antibody (SAA0143)
Brand: abinScience
SKU: HC513107
Price: 191 USD
Availability: InStock

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Vista general

Número de catálogo	HC513107
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG2, kappa
ID de clon	SAA0143
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	Q04721, Q9UM47
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Neurogenic locus notch homolog protein 2 (NOTCH2/NOTCH3) is a ~265 kDa protein. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. 1. Simpson, MA. et al. (2011) Nature genetics 43, 303-5. PMID: 21378985 2. Isidor, B. et al. (2011) Nature genetics 43, 306-8. PMID: 21378989 3. Fukushima, H. et al. (2017) Molecular cell 68, 645-658.e5. PMID: 29149593 4. Zhu, P. et al. (2015) Nature communications 6, 7122. PMID: 25985737
Nota	For research use only.

Imágenes

Flow Cytometry

Flow-cytometry using anti-human NOTCH2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human NOTCH3 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH3 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human NOTCH2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human NOTCH2 & NOTCH3 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 & NOTCH3 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-Human Notch-2/Notch-3 Recombinant Antibody (SAA0143).pdf

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