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Каталожный номерHP062014
Описание
Anti-Human NXF1 Polyclonal Antibody (HP062014) is a rabbit polyclonal antibody detecting NXF1 in ELISA, IHC, WB. Suitable for Human, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human NXF1 (Lys205-Lys347).
Цель mRNA export factor TAP, Nuclear RNA export factor 1, Tip-associating protein, TAP, Tip-associated protein, NXF1
Очистка Purified by antigen affinity column.
Номер доступа Q9UBU9
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Nuclear RNA export factor 1 (NXF1) is a ~70 kDa protein. Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.

1. Tang, H. et al. (2000) The Journal of biological chemistry 275, 32694-700. PMID: 10924507
2. Hautbergue, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5154-9. PMID: 18364396
3. Katahira, J. et al. (2009) The EMBO journal 28, 556-67. PMID: 19165146
4. Grüter, P. et al. (1998) Molecular cell 1, 649-59. PMID: 9660949
5. Roundtree, IA. et al. (2017) eLife 6. PMID: 28984244
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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