Human NXF1 Recombinant Protein (N-His) (HP062012)

Name: Human NXF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP062012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP062012
Description	Recombinant Human NXF1 Protein, N-His (HP062012) expressed in E. coli, spanning Lys205-Lys347. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9UBU9
Protein length	Lys205-Lys347
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.71 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	mRNA export factor TAP, Nuclear RNA export factor 1, Tip-associating protein, TAP, Tip-associated protein, NXF1
Background	Nuclear RNA export factor 1 (NXF1) is a ~70 kDa protein. Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. 1. Tang, H. et al. (2000) The Journal of biological chemistry 275, 32694-700. PMID: 10924507 2. Hautbergue, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5154-9. PMID: 18364396 3. Katahira, J. et al. (2009) The EMBO journal 28, 556-67. PMID: 19165146 4. Grüter, P. et al. (1998) Molecular cell 1, 649-59. PMID: 9660949 5. Roundtree, IA. et al. (2017) eLife 6. PMID: 28984244
Note	For research use only.

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SDS-PAGE for Recombinant Human NXF1 Protein, N-His

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Datasheet

Human NXF1 Recombinant Protein (N-His).pdf

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Human NXF1 Recombinant Protein (N-His) (HP062012)

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