Anti-Human NXF1 Polyclonal Antibody (HP062014)

Name: Anti-Human NXF1 Polyclonal Antibody
Brand: abinScience
SKU: HP062014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP062014

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NXF1 (Lys205-Lys347).

Target

mRNA export factor TAP, Nuclear RNA export factor 1, Tip-associating protein, TAP, Tip-associated protein, NXF1

Purification

Purified by antigen affinity column.

Accession

Q9UBU9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nuclear RNA export factor 1 (NXF1) is a ~70 kDa protein. Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.

1. Tang, H. et al. (2000) The Journal of biological chemistry 275, 32694-700. PMID: 10924507

2. Hautbergue, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5154-9. PMID: 18364396

3. Katahira, J. et al. (2009) The EMBO journal 28, 556-67. PMID: 19165146

4. Grüter, P. et al. (1998) Molecular cell 1, 649-59. PMID: 9660949

5. Roundtree, IA. et al. (2017) eLife 6. PMID: 28984244

Note

For research use only.

Images

References

Datasheet

Anti-Human NXF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human NXF1 Polyclonal Antibody (HP062014)

Overview

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References

Datasheet

SDS

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