Anti-Human Osteopontin/OPN Recombinant Antibody (SAA0512) (HY207107)

Name: Anti-Human Osteopontin/OPN Recombinant Antibody (SAA0512)
Brand: abinScience
SKU: HY207107
Price: 191 USD
Availability: InStock

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HY207107
Reactividad de especies	Human
Aplicaciones	ELISA, FCM, SPR, WB
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG2a, kappa
ID de clon	SAA0512
Objetivo	Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P10451
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Osteopontin (OPN/SPP1) is a ~35 kDa protein. Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human OPN/SPP1 Antibody (SAA0512)
Flow Cytometry

Flow-cytometry using anti-human OPN antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # HY207107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OPN/SPP1 (Catalog No: HY207012)
Lane 2: negative control

Referencias

Datasheet

Anti-Human Osteopontin/OPN Recombinant Antibody (SAA0512).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

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