





| Numéro de catalogue | HY207107 |
|---|---|
| Reactivité des espèces | Human |
| Applications | ELISA, FCM, SPR, WB |
| Espèce hôte | Mouse |
| Clonalité | Monoclonal |
| Isotype | IgG2a, kappa |
| ID de clone | SAA0512 |
| Cible | Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1 |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Numéro d'accès | P10451 |
| Forme | Liquid |
| Tampon de stockage | 0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Contexte | Osteopontin (OPN/SPP1) is a ~35 kDa protein. Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. |
| Note | For research use only. |

SDS-PAGE for Anti-Human OPN/SPP1 Antibody (SAA0512)

Flow-cytometry using anti-human OPN antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # HY207107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OPN/SPP1 (Catalog No: HY207012)
Lane 2: negative control




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