

| Numéro de catalogue | HD130014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Description |
Anti-Human ORF2p Polyclonal Antibody (HD130014) is a rabbit polyclonal antibody detecting ORF2p. Suitable for Human.
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| Reactivité des espèces | Human | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Espèce hôte | Rabbit | ||||||||
| Clonalité | Polyclonal | ||||||||
| Isotype | IgG | ||||||||
| Immunogène | E. coli - derived recombinant Human ORF2p (Met1-Lys240). | ||||||||
| Cible | EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase | ||||||||
| Niveau d'endotoxines | Please contact with the lab for this information. | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Numéro d'accès | O00370 | ||||||||
| Forme | Liquid | ||||||||
| Tampon de stockage | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
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| Informations d'utilisation du produit |
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| Contexte | LINE-1 retrotransposable element ORF2 protein (ORF2p) is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition. 1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901 2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902 3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468 4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393 5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046 6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261 7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517 | ||||||||
| Note | For research use only |
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