Human ORF2p Recombinant Protein (N-His) (HD130012)

Name: Human ORF2p Recombinant Protein (N-His)
Brand: abinScience
SKU: HD130012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD130012
Description	Recombinant Human ORF2p Protein, N-His (HD130012) expressed in E. coli, spanning Met1-Lys240. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O00370
Protein length	Met1-Lys240
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.56 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase
Background	LINE-1 retrotransposable element ORF2 protein is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition. 1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901 2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902 3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468 4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393 5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046 6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261 7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517
Note	For research use only

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Datasheet

Human ORF2p Recombinant Protein (N-His).pdf

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Human ORF2p Recombinant Protein (N-His) (HD130012)

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