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Anti-Human ORF2p Polyclonal Antibody (HD130014)

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Overview
Catalog No.HD130014
Description
Anti-Human ORF2p Polyclonal Antibody (HD130014) is a rabbit polyclonal antibody detecting ORF2p. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ORF2p (Met1-Lys240).
Target EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O00370
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

LINE-1 retrotransposable element ORF2 protein (ORF2p) is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition.

1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901
2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902
3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468
4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393
5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046
6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261
7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517
Note For research use only
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Formula
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Anti-Human ORF2p Polyclonal Antibody

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