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Anti-Human OS9 Polyclonal Antibody (HC357014)

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Vista general
Número de catálogoHC357014
Descripción
Anti-Human OS9 Polyclonal Antibody (HC357014) is a rabbit polyclonal antibody detecting OS9 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human OS9 (Asn32-Gln251).
Objetivo Protein OS-9, OS9, Amplified in osteosarcoma 9
Purificación Purified by antigen affinity column.
Número de acceso Q13438
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Protein OS-9 (OS9) is a ~75 kDa protein. Lectin component of the HRD1 complex, which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). Specifically recognizes and binds improperly folded glycoproteins as well as hyperglycosylated proteins, retain them in the ER, and transfers them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 as well as hyperglycosylated HSP90B1.

1. Christianson, JC. et al. (2008) Nature cell biology 10, 272-82. PMID: 18264092
2. Bernasconi, R. et al. (2008) The Journal of biological chemistry 283, 16446-54. PMID: 18417469
3. Alcock, F. et al. (2009) Journal of molecular biology 385, 1032-42. PMID: 19084021
4. Hosokawa, N. et al. (2009) The Journal of biological chemistry 284, 17061-17068. PMID: 19346256
5. Satoh, T. et al. (2010) Molecular cell 40, 905-16. PMID: 21172656
6. Dersh, D. et al. (2014) Molecular biology of the cell 25, 2220-34. PMID: 24899641
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human OS9 Polyclonal Antibody

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