Human OS9 Recombinant Protein (N-His) (HC357012)

Name: Human OS9 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC357012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC357012
Description	Recombinant Human OS9 Protein, N-His (HC357012) expressed in E. coli, spanning Asn32-Gln251. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13438
Protein length	Asn32-Gln251
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	27.48 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein OS-9, OS9, Amplified in osteosarcoma 9
Background	Protein OS-9 (OS9) is a ~75 kDa protein. Lectin component of the HRD1 complex, which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). Specifically recognizes and binds improperly folded glycoproteins as well as hyperglycosylated proteins, retain them in the ER, and transfers them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 as well as hyperglycosylated HSP90B1. 1. Christianson, JC. et al. (2008) Nature cell biology 10, 272-82. PMID: 18264092 2. Bernasconi, R. et al. (2008) The Journal of biological chemistry 283, 16446-54. PMID: 18417469 3. Alcock, F. et al. (2009) Journal of molecular biology 385, 1032-42. PMID: 19084021 4. Hosokawa, N. et al. (2009) The Journal of biological chemistry 284, 17061-17068. PMID: 19346256 5. Satoh, T. et al. (2010) Molecular cell 40, 905-16. PMID: 21172656 6. Dersh, D. et al. (2014) Molecular biology of the cell 25, 2220-34. PMID: 24899641
Note	For research use only.

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SDS-PAGE for Recombinant Human OS9 Protein, N-His

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Datasheet

Human OS9 Recombinant Protein (N-His).pdf

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Human OS9 Recombinant Protein (N-His) (HC357012)

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