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Anti-Human OS9 Polyclonal Antibody (HC357014)

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Aperçu
Numéro de catalogueHC357014
Description
Anti-Human OS9 Polyclonal Antibody (HC357014) is a rabbit polyclonal antibody detecting OS9 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human OS9 (Asn32-Gln251).
Cible Protein OS-9, OS9, Amplified in osteosarcoma 9
Purification Purified by antigen affinity column.
Numéro d'accès Q13438
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein OS-9 (OS9) is a ~75 kDa protein. Lectin component of the HRD1 complex, which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). Specifically recognizes and binds improperly folded glycoproteins as well as hyperglycosylated proteins, retain them in the ER, and transfers them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 as well as hyperglycosylated HSP90B1.

1. Christianson, JC. et al. (2008) Nature cell biology 10, 272-82. PMID: 18264092
2. Bernasconi, R. et al. (2008) The Journal of biological chemistry 283, 16446-54. PMID: 18417469
3. Alcock, F. et al. (2009) Journal of molecular biology 385, 1032-42. PMID: 19084021
4. Hosokawa, N. et al. (2009) The Journal of biological chemistry 284, 17061-17068. PMID: 19346256
5. Satoh, T. et al. (2010) Molecular cell 40, 905-16. PMID: 21172656
6. Dersh, D. et al. (2014) Molecular biology of the cell 25, 2220-34. PMID: 24899641
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human OS9 Polyclonal Antibody

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