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Anti-Human PIP4K2A Polyclonal Antibody (HD637014)

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Aperçu
Numéro de catalogueHD637014
Description
Anti-Human PIP4K2A Polyclonal Antibody (HD637014) is a rabbit polyclonal antibody detecting PIP4K2A. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PIP4K2A (Ser34-Thr406).
Cible 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha, Diphosphoinositide kinase 2-alpha, EC:2.7.1.149, PI(5)P 4-kinase type II alpha, PI5P4KA, PI5P4Kalpha, PIP4K2A, PIP4KII-alpha, PIP5K2, PIP5K2A, PIP5KIII, Phosphatidylinositol 5-Phosphate 4-Kinase, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PtdIns(5)P-4-kinase isoform 2-alpha
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P48426
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a ~46 kDa protein. Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Has both ATP- and GTP-dependent kinase activities. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level.

1. Davis, MI. et al. (2013) PloS one 8, e54127. PMID: 23326584
2. Rameh, LE. et al. (1997) Nature 390, 192-6. PMID: 9367159
3. Sumita, K. et al. (2016) Molecular cell 61, 187-98. PMID: 26774281
4. Wilcox, A. et al. (2008) FEBS letters 582, 1391-4. PMID: 18364242
5. Hu, A. et al. (2018) Journal of lipid research 59, 507-514. PMID: 29353240
6. Wang, DG. et al. (2019) Cell reports 27, 1991-2001.e5. PMID: 31091439
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human PIP4K2A Polyclonal Antibody

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