ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha, Diphosphoinositide kinase 2-alpha, EC:2.7.1.149, PI(5)P 4-kinase type II alpha, PI5P4KA, PI5P4Kalpha, PIP4K2A, PIP4KII-alpha, PIP5K2, PIP5K2A, PIP5KIII, Phosphatidylinositol 5-Phosphate 4-Kinase, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PtdIns(5)P-4-kinase isoform 2-alpha
Background
Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha is a ~46 kDa protein. Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Has both ATP- and GTP-dependent kinase activities. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level.
1. Davis, MI. et al. (2013) PloS one 8, e54127. PMID: 23326584
2. Rameh, LE. et al. (1997) Nature 390, 192-6. PMID: 9367159
3. Sumita, K. et al. (2016) Molecular cell 61, 187-98. PMID: 26774281
4. Wilcox, A. et al. (2008) FEBS letters 582, 1391-4. PMID: 18364242
5. Hu, A. et al. (2018) Journal of lipid research 59, 507-514. PMID: 29353240
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