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Anti-Human POLD4 Polyclonal Antibody (HD558014)

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Aperçu
Numéro de catalogueHD558014
Description
Anti-Human POLD4 Polyclonal Antibody (HD558014) is a rabbit polyclonal antibody detecting POLD4. Suitable for Human.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human POLD4 (Glu45-Leu107).
Cible DNA polymerase delta subunit 4, DNA polymerase delta subunit p12, POLD4, POLDS
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q9HCU8
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

DNA polymerase delta subunit 4 (POLD4) is a ~12 kDa protein. As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites.

1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448
2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196
3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433
4. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200
5. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611
6. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human POLD4 Polyclonal Antibody

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