Human POLD4 Recombinant Protein (N-GST & C-His) (HD558012)

Name: Human POLD4 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HD558012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD558012
Description	Recombinant Human POLD4 Protein, N-GST & C-His (HD558012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q9HCU8
Protein length	Glu45-Leu107
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA polymerase delta subunit 4, DNA polymerase delta subunit p12, POLD4, POLDS
Background	DNA polymerase delta subunit 4 is a ~12 kDa protein. As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. 1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448 2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196 3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433 4. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200 5. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611 6. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480
Note	For research use only

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Datasheet

Human POLD4 Recombinant Protein (N-GST & C-His).pdf

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Human POLD4 Recombinant Protein (N-GST & C-His) (HD558012)

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