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Anti-Human POLG Polyclonal Antibody (HX196014)

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Aperçu
Numéro de catalogueHX196014
Description
Anti-Human POLG Polyclonal Antibody (HX196014) is a rabbit polyclonal antibody detecting POLG. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human POLG (Trp1041-Pro1239).
Cible 3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-, EC:4.2.99.-, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLG1, POLGA, PolG-alpha
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P54098
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

DNA polymerase subunit gamma-1 (POLG) is a ~139 kDa protein. Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates. Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site.

1. Johnson, AA. et al. (2001) The Journal of biological chemistry 276, 38090-6. PMID: 11477093
2. Ponamarev, MV. et al. (2002) The Journal of biological chemistry 277, 15225-8. PMID: 11897778
3. Luoma, PT. et al. (2005) Human molecular genetics 14, 1907-20. PMID: 15917273
4. Lee, YS. et al. (2009) Cell 139, 312-24. PMID: 19837034
5. Graves, SW. et al. (1998) Biochemistry 37, 6050-8. PMID: 9558343
6. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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