Human POLG Recombinant Protein (N-His) (HX196012)

Name: Human POLG Recombinant Protein (N-His)
Brand: abinScience
SKU: HX196012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX196012
Description	Recombinant Human POLG Protein, N-His (HX196012) expressed in E. coli, spanning Trp1041-Pro1239. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P54098
Protein length	Trp1041-Pro1239
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.97 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	POLG, Mitochondrial DNA polymerase catalytic subunit, POLGA, PolG-alpha, POLG1, DNA polymerase subunit gamma-1, MDP1
Background	DNA polymerase subunit gamma-1 (POLG) is a ~139 kDa protein. Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates. Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site. 1. Johnson, AA. et al. (2001) The Journal of biological chemistry 276, 38090-6. PMID: 11477093 2. Ponamarev, MV. et al. (2002) The Journal of biological chemistry 277, 15225-8. PMID: 11897778 3. Luoma, PT. et al. (2005) Human molecular genetics 14, 1907-20. PMID: 15917273 4. Lee, YS. et al. (2009) Cell 139, 312-24. PMID: 19837034 5. Graves, SW. et al. (1998) Biochemistry 37, 6050-8. PMID: 9558343 6. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897
Note	For research use only.

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SDS-PAGE for Recombinant Human POLG Protein, N-His

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Datasheet

Human POLG Recombinant Protein (N-His).pdf

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Human POLG Recombinant Protein (N-His) (HX196012)

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