Anti-Human POLG Polyclonal Antibody (HX196014)

Name: Anti-Human POLG Polyclonal Antibody
Brand: abinScience
SKU: HX196014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HX196014

Description

Anti-Human POLG Polyclonal Antibody (HX196014) is a rabbit polyclonal antibody detecting POLG. Suitable for Human, Rat, and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POLG (Trp1041-Pro1239).

Target

3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-, EC:4.2.99.-, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLG1, POLGA, PolG-alpha

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P54098

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

DNA polymerase subunit gamma-1 (POLG) is a ~139 kDa protein. Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates. Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site.

1. Johnson, AA. et al. (2001) The Journal of biological chemistry 276, 38090-6. PMID: 11477093

2. Ponamarev, MV. et al. (2002) The Journal of biological chemistry 277, 15225-8. PMID: 11897778

3. Luoma, PT. et al. (2005) Human molecular genetics 14, 1907-20. PMID: 15917273

4. Lee, YS. et al. (2009) Cell 139, 312-24. PMID: 19837034

5. Graves, SW. et al. (1998) Biochemistry 37, 6050-8. PMID: 9558343

6. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

Note

For research use only

Images

References

Datasheet

Anti-Human POLG Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human POLG Polyclonal Antibody (HX196014)

Overview

Images

References

Datasheet

SDS

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