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| カタログ番号 | HX196014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human POLG Polyclonal Antibody (HX196014) is a rabbit polyclonal antibody detecting POLG. Suitable for Human, Rat, and Mouse.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human POLG (Trp1041-Pro1239). | ||||||||
| ターゲット | 3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-, EC:4.2.99.-, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLG1, POLGA, PolG-alpha | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P54098 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 背景 | DNA polymerase subunit gamma-1 (POLG) is a ~139 kDa protein. Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates. Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site. 1. Johnson, AA. et al. (2001) The Journal of biological chemistry 276, 38090-6. PMID: 11477093 2. Ponamarev, MV. et al. (2002) The Journal of biological chemistry 277, 15225-8. PMID: 11897778 3. Luoma, PT. et al. (2005) Human molecular genetics 14, 1907-20. PMID: 15917273 4. Lee, YS. et al. (2009) Cell 139, 312-24. PMID: 19837034 5. Graves, SW. et al. (1998) Biochemistry 37, 6050-8. PMID: 9558343 6. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897 | ||||||||
| 注意事項 | For research use only |
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