Anti-Human RAD1 Polyclonal Antibody (HT370014)

Name: Anti-Human RAD1 Polyclonal Antibody
Brand: abinScience
SKU: HT370014
Price: 168 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HT370014

Description

Anti-Human RAD1 Polyclonal Antibody (HT370014) is a rabbit polyclonal antibody detecting RAD1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human RAD1 (Met1-Ser282).

Cible

DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1, hRAD1, Cell cycle checkpoint protein RAD1

Purification

Purified by antigen affinity column.

Numéro d'accès

O60671

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Cell cycle checkpoint protein RAD1 is a ~31 kDa protein. Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

1. Cai, RL. et al. (2000) The Journal of biological chemistry 275, 27909-16. PMID: 10846170

2. Rauen, M. et al. (2000) The Journal of biological chemistry 275, 29767-71. PMID: 10884395

3. Bermudez, VP. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1633-8. PMID: 12578958

4. Smirnova, E. et al. (2005) The Biochemical journal 389, 13-7. PMID: 15871698

5. Toueille, M. et al. (2004) Nucleic acids research 32, 3316-24. PMID: 15314187

6. Wang, W. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 16762-7. PMID: 15556996

7. Cotta-Ramusino, C. et al. (2011) Science (New York, N.Y.) 332, 1313-7. PMID: 21659603

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human RAD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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Kits d'Essai

Anti-Human RAD1 Polyclonal Antibody (HT370014)

Aperçu

Images

Reférences

Fiche technique

SDS