Human RAD1 Recombinant Protein (N-His) (HT370012)

Name: Human RAD1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT370012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT370012
Description	Recombinant Human RAD1 Protein, N-His (HT370012) expressed in E. coli, spanning Met1-Ser282. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60671
Protein length	Met1-Ser282
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.99 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1, hRAD1, Cell cycle checkpoint protein RAD1
Background	Cell cycle checkpoint protein RAD1 is a ~31 kDa protein. Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. 1. Cai, RL. et al. (2000) The Journal of biological chemistry 275, 27909-16. PMID: 10846170 2. Rauen, M. et al. (2000) The Journal of biological chemistry 275, 29767-71. PMID: 10884395 3. Bermudez, VP. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1633-8. PMID: 12578958 4. Smirnova, E. et al. (2005) The Biochemical journal 389, 13-7. PMID: 15871698 5. Toueille, M. et al. (2004) Nucleic acids research 32, 3316-24. PMID: 15314187 6. Wang, W. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 16762-7. PMID: 15556996 7. Cotta-Ramusino, C. et al. (2011) Science (New York, N.Y.) 332, 1313-7. PMID: 21659603
Note	For research use only.

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SDS-PAGE for Recombinant Human RAD1 Protein, N-His

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Datasheet

Human RAD1 Recombinant Protein (N-His).pdf

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Human RAD1 Recombinant Protein (N-His) (HT370012)

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