Anti-Human RAD1 Polyclonal Antibody (HT370014)

Name: Anti-Human RAD1 Polyclonal Antibody
Brand: abinScience
SKU: HT370014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT370014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAD1 (Met1-Ser282).

Target

DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1, hRAD1, Cell cycle checkpoint protein RAD1

Purification

Purified by antigen affinity column.

Accession

O60671

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell cycle checkpoint protein RAD1 is a ~31 kDa protein. Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

1. Cai, RL. et al. (2000) The Journal of biological chemistry 275, 27909-16. PMID: 10846170

2. Rauen, M. et al. (2000) The Journal of biological chemistry 275, 29767-71. PMID: 10884395

3. Bermudez, VP. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1633-8. PMID: 12578958

4. Smirnova, E. et al. (2005) The Biochemical journal 389, 13-7. PMID: 15871698

5. Toueille, M. et al. (2004) Nucleic acids research 32, 3316-24. PMID: 15314187

6. Wang, W. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 16762-7. PMID: 15556996

7. Cotta-Ramusino, C. et al. (2011) Science (New York, N.Y.) 332, 1313-7. PMID: 21659603

Note

For research use only.

Images

References

Datasheet

Anti-Human RAD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RAD1 Polyclonal Antibody (HT370014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us