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Anti-Human RAD51AP1 Polyclonal Antibody (HP402014)

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Aperçu
Numéro de catalogueHP402014
Description
Anti-Human RAD51AP1 Polyclonal Antibody (HP402014) is a rabbit polyclonal antibody detecting RAD51AP1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human RAD51AP1 (Arg70-Asn116).
Cible PIR51, RAD51-interacting protein, RAD51-associated protein 1, RAD51AP1, HsRAD51AP1
Purification Purified by antigen affinity column.
Numéro d'accès Q96B01
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

RAD51-associated protein 1 (RAD51AP1) is a ~38 kDa protein. Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2.

1. Modesti, M. et al. (2007) Molecular cell 28, 468-81. PMID: 17996710
2. Wiese, C. et al. (2007) Molecular cell 28, 482-90. PMID: 17996711
3. Dray, E. et al. (2010) Nature structural & molecular biology 17, 1255-9. PMID: 20871616
4. Parplys, AC. et al. (2014) DNA repair 24, 87-97. PMID: 25288561
5. Parplys, AC. et al. (2015) Nucleic acids research 43, 9817-34. PMID: 26323318
6. Dunlop, MH. et al. (2012) The Journal of biological chemistry 287, 12343-7. PMID: 22375013
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human RAD51AP1 Polyclonal Antibody

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