Human RAD51AP1 Recombinant Protein (N-His-SUMO) (HP402012)

Name: Human RAD51AP1 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HP402012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP402012
Description	Recombinant Human RAD51AP1 Protein, N-His-SUMO (HP402012) expressed in E. coli, spanning Arg70-Asn116. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96B01
Protein length	Arg70-Asn116
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.45 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PIR51, RAD51-interacting protein, RAD51-associated protein 1, RAD51AP1, HsRAD51AP1
Background	RAD51-associated protein 1 (RAD51AP1) is a ~38 kDa protein. Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. 1. Modesti, M. et al. (2007) Molecular cell 28, 468-81. PMID: 17996710 2. Wiese, C. et al. (2007) Molecular cell 28, 482-90. PMID: 17996711 3. Dray, E. et al. (2010) Nature structural & molecular biology 17, 1255-9. PMID: 20871616 4. Parplys, AC. et al. (2014) DNA repair 24, 87-97. PMID: 25288561 5. Parplys, AC. et al. (2015) Nucleic acids research 43, 9817-34. PMID: 26323318 6. Dunlop, MH. et al. (2012) The Journal of biological chemistry 287, 12343-7. PMID: 22375013
Note	For research use only.

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SDS-PAGE for Recombinant Human RAD51AP1 Protein, N-His-SUMO

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Datasheet

Human RAD51AP1 Recombinant Protein (N-His-SUMO).pdf

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Human RAD51AP1 Recombinant Protein (N-His-SUMO) (HP402012)

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