Anti-Human RAD51AP1 Polyclonal Antibody (HP402014)

Name: Anti-Human RAD51AP1 Polyclonal Antibody
Brand: abinScience
SKU: HP402014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP402014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAD51AP1 (Arg70-Asn116).

Target

PIR51, RAD51-interacting protein, RAD51-associated protein 1, RAD51AP1, HsRAD51AP1

Purification

Purified by antigen affinity column.

Accession

Q96B01

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RAD51-associated protein 1 (RAD51AP1) is a ~38 kDa protein. Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2.

1. Modesti, M. et al. (2007) Molecular cell 28, 468-81. PMID: 17996710

2. Wiese, C. et al. (2007) Molecular cell 28, 482-90. PMID: 17996711

3. Dray, E. et al. (2010) Nature structural & molecular biology 17, 1255-9. PMID: 20871616

4. Parplys, AC. et al. (2014) DNA repair 24, 87-97. PMID: 25288561

5. Parplys, AC. et al. (2015) Nucleic acids research 43, 9817-34. PMID: 26323318

6. Dunlop, MH. et al. (2012) The Journal of biological chemistry 287, 12343-7. PMID: 22375013

Note

For research use only.

Images

References

Datasheet

Anti-Human RAD51AP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RAD51AP1 Polyclonal Antibody (HP402014)

Overview

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References

Datasheet

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