Anti-Human RHEB Polyclonal Antibody (HS914014)

Name: Anti-Human RHEB Polyclonal Antibody
Brand: abinScience
SKU: HS914014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HS914014

Описание

Anti-Human RHEB Polyclonal Antibody (HS914014) is a rabbit polyclonal antibody detecting RHEB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human RHEB (Met1-Met184).

Цель

RHEB2, Ras homolog enriched in brain, RHEB, GTP-binding protein Rheb

Очистка

Purified by antigen affinity column.

Номер доступа

Q15382

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

GTP-binding protein Rheb is a ~20 kDa protein. Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity.

1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553

2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141

3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888

4. Inoki, K. et al. (2003) Genes & development 17, 1829-34. PMID: 12869586

5. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785

6. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059

7. Long, X. et al. (2005) Current biology : CB 15, 702-13. PMID: 15854902

8. Tee, AR. et al. (2005) FEBS letters 579, 4763-8. PMID: 16098514

9. Sancak, Y. et al. (2010) Cell 141, 290-303. PMID: 20381137

10. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human RHEB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа