ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
22.66 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
RHEB2, Ras homolog enriched in brain, RHEB, GTP-binding protein Rheb
Background
GTP-binding protein Rheb is a ~20 kDa protein. Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity.
1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553
2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141
3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888
4. Inoki, K. et al. (2003) Genes & development 17, 1829-34. PMID: 12869586
5. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785
6. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059
7. Long, X. et al. (2005) Current biology : CB 15, 702-13. PMID: 15854902
8. Tee, AR. et al. (2005) FEBS letters 579, 4763-8. PMID: 16098514
9. Sancak, Y. et al. (2010) Cell 141, 290-303. PMID: 20381137
10. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human RHEB Protein, N-His
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