Anti-Human RHOA Polyclonal Antibody (HX184014)

Name: Anti-Human RHOA Polyclonal Antibody
Brand: abinScience
SKU: HX184014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HX184014

Description

Anti-Human RHOA Polyclonal Antibody (HX184014) is a rabbit polyclonal antibody detecting RHOA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human RHOA (Ala2-Gly189).

Cible

Rho cDNA clone 12, ARH12, RHO12, ARHA, RHOA, Transforming protein RhoA, h12

Purification

Purified by antigen affinity column.

Numéro d'accès

P61586

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Transforming protein RhoA is a ~21 kDa protein. Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation.

1. Wei, J. et al. (2013) Biochimica et biophysica acta 1833, 2757-2764. PMID: 23871831

2. Vabres, P. et al. (2019) Nature genetics 51, 1438-1441. PMID: 31570889

3. Quilliam, LA. et al. (1996) The Journal of biological chemistry 271, 28772-6. PMID: 8910519

4. Vincent, S. et al. (1997) Molecular and cellular biology 17, 2247-56. PMID: 9121475

5. Wing, MR. et al. (2003) The Journal of biological chemistry 278, 41253-8. PMID: 12900402

6. Kamijo, K. et al. (2006) Molecular biology of the cell 17, 43-55. PMID: 16236794

7. Wallace, SW. et al. (2011) Molecular and cellular biology 31, 81-91. PMID: 20974804

8. Dubash, AD. et al. (2013) The Journal of cell biology 202, 653-66. PMID: 23940119

9. Bristow, JM. et al. (2009) Journal of cell science 122, 4535-46. PMID: 19934221

10. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human RHOA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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