ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
26.21 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Transforming protein RhoA is a ~21 kDa protein. Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation.
1. Wei, J. et al. (2013) Biochimica et biophysica acta 1833, 2757-2764. PMID: 23871831
2. Vabres, P. et al. (2019) Nature genetics 51, 1438-1441. PMID: 31570889
3. Quilliam, LA. et al. (1996) The Journal of biological chemistry 271, 28772-6. PMID: 8910519
4. Vincent, S. et al. (1997) Molecular and cellular biology 17, 2247-56. PMID: 9121475
5. Wing, MR. et al. (2003) The Journal of biological chemistry 278, 41253-8. PMID: 12900402
6. Kamijo, K. et al. (2006) Molecular biology of the cell 17, 43-55. PMID: 16236794
7. Wallace, SW. et al. (2011) Molecular and cellular biology 31, 81-91. PMID: 20974804
8. Dubash, AD. et al. (2013) The Journal of cell biology 202, 653-66. PMID: 23940119
9. Bristow, JM. et al. (2009) Journal of cell science 122, 4535-46. PMID: 19934221
10. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human RHOA Protein, N-Strep
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