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Anti-Human RHOA Polyclonal Antibody (HX184014)

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Overview
Catalog No.HX184014
Description
Anti-Human RHOA Polyclonal Antibody (HX184014) is a rabbit polyclonal antibody detecting RHOA in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RHOA (Ala2-Gly189).
Target Rho cDNA clone 12, ARH12, RHO12, ARHA, RHOA, Transforming protein RhoA, h12
Purification Purified by antigen affinity column.
Accession P61586
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Transforming protein RhoA is a ~21 kDa protein. Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation.

1. Wei, J. et al. (2013) Biochimica et biophysica acta 1833, 2757-2764. PMID: 23871831
2. Vabres, P. et al. (2019) Nature genetics 51, 1438-1441. PMID: 31570889
3. Quilliam, LA. et al. (1996) The Journal of biological chemistry 271, 28772-6. PMID: 8910519
4. Vincent, S. et al. (1997) Molecular and cellular biology 17, 2247-56. PMID: 9121475
5. Wing, MR. et al. (2003) The Journal of biological chemistry 278, 41253-8. PMID: 12900402
6. Kamijo, K. et al. (2006) Molecular biology of the cell 17, 43-55. PMID: 16236794
7. Wallace, SW. et al. (2011) Molecular and cellular biology 31, 81-91. PMID: 20974804
8. Dubash, AD. et al. (2013) The Journal of cell biology 202, 653-66. PMID: 23940119
9. Bristow, JM. et al. (2009) Journal of cell science 122, 4535-46. PMID: 19934221
10. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human RHOA Polyclonal Antibody

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