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| カタログ番号 | HA155014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human RTRAF Polyclonal Antibody (HA155014) is a rabbit polyclonal antibody detecting RTRAF in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human RTRAF (Phe2-Glu118). | ||||||||
| ターゲット | RNA transcription, translation and transport factor protein, CLE7 homolog, CLE, hCLE, RTRAF, C14orf166, CGI-99 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9Y224 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | tRNA-splicing ligase complex subunit RTRAF is a ~28 kDa protein. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport. 1. Popow, J. et al. (2011) Science (New York, N.Y.) 331, 760-4. PMID: 21311021 2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230 3. Pérez-González, A. et al. (2006) Journal of molecular biology 362, 887-900. PMID: 16950395 4. Pérez-González, A. et al. (2014) PloS one 9, e90957. PMID: 24608264 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-RTRAF polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
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