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Human RTRAF Recombinant Protein (N-His) (HA155022)

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Overview
Catalog No.HA155022
Description
Recombinant Human RTRAF Protein, N-His (HA155022) expressed in E. coli, spanning Met1-Glu118. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9Y224
Protein lengthMet1-Glu118
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 16.28 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesC14orf166, CLE, CLE7 homolog, RNA transcription, translation and transport factor protein, RTRAF, hCLE
Background

tRNA-splicing ligase complex subunit RTRAF is a ~28 kDa protein. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport.

1. Popow, J. et al. (2011) Science (New York, N.Y.) 331, 760-4. PMID: 21311021
2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230
3. Pérez-González, A. et al. (2006) Journal of molecular biology 362, 887-900. PMID: 16950395
4. Pérez-González, A. et al. (2014) PloS one 9, e90957. PMID: 24608264
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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