

| Catalog No. | HA155022 |
|---|---|
| Description |
Recombinant Human RTRAF Protein, N-His (HA155022) expressed in E. coli, spanning Met1-Glu118. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | Q9Y224 |
| Protein length | Met1-Glu118 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 16.28 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | C14orf166, CLE, CLE7 homolog, RNA transcription, translation and transport factor protein, RTRAF, hCLE |
| Background | tRNA-splicing ligase complex subunit RTRAF is a ~28 kDa protein. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport. 1. Popow, J. et al. (2011) Science (New York, N.Y.) 331, 760-4. PMID: 21311021 2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230 3. Pérez-González, A. et al. (2006) Journal of molecular biology 362, 887-900. PMID: 16950395 4. Pérez-González, A. et al. (2014) PloS one 9, e90957. PMID: 24608264 |
| Note | For research use only |
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