Anti-Human RTRAF Polyclonal Antibody (HA155014)

Name: Anti-Human RTRAF Polyclonal Antibody
Brand: abinScience
SKU: HA155014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA155014

Description

Anti-Human RTRAF Polyclonal Antibody (HA155014) is a rabbit polyclonal antibody detecting RTRAF in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RTRAF (Phe2-Glu118).

Target

RNA transcription, translation and transport factor protein, CLE7 homolog, CLE, hCLE, RTRAF, C14orf166, CGI-99

Purification

Purified by antigen affinity column.

Accession

Q9Y224

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

tRNA-splicing ligase complex subunit RTRAF is a ~28 kDa protein. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport.

1. Popow, J. et al. (2011) Science (New York, N.Y.) 331, 760-4. PMID: 21311021

2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230

3. Pérez-González, A. et al. (2006) Journal of molecular biology 362, 887-900. PMID: 16950395

4. Pérez-González, A. et al. (2014) PloS one 9, e90957. PMID: 24608264

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RTRAF polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate

References

Datasheet

Anti-Human RTRAF Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RTRAF Polyclonal Antibody (HA155014)

Overview

Images

References

Datasheet

SDS

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