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Anti-Human RTRAF Polyclonal Antibody
Anti-Human RTRAF Polyclonal Antibody
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHA155014
Описание
Anti-Human RTRAF Polyclonal Antibody (HA155014) is a rabbit polyclonal antibody detecting RTRAF in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human RTRAF (Phe2-Glu118).
Цель RNA transcription, translation and transport factor protein, CLE7 homolog, CLE, hCLE, RTRAF, C14orf166, CGI-99
Очистка Purified by antigen affinity column.
Номер доступа Q9Y224
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

tRNA-splicing ligase complex subunit RTRAF is a ~28 kDa protein. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport.

1. Popow, J. et al. (2011) Science (New York, N.Y.) 331, 760-4. PMID: 21311021
2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230
3. Pérez-González, A. et al. (2006) Journal of molecular biology 362, 887-900. PMID: 16950395
4. Pérez-González, A. et al. (2014) PloS one 9, e90957. PMID: 24608264
Примечание For research use only.
Изображения
  • Anti-Human RTRAF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RTRAF polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: 293T cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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