Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228) (HT245013)

Name: Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228)
Brand: abinScience
SKU: HT245013
Price: 191 USD
Availability: InStock

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Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HT245013

Reactivité des espèces

Human, Mouse

Applications

ELISA, IF, IP

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

SAA0228

Cible

PRMT5, SKB1 homolog, 72 kDa ICln-binding protein, Jak-binding protein 1, Histone-arginine N-methyltransferase PRMT5, SKB1Hs, JBP1, Shk1 kinase-binding protein 1 homolog, SKB1, HRMT1L5, IBP72, Protein arginine N-methyltransferase 5

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

O14744

RRID

Anti-Human SKB1/PRMT5 IP Antibody (SAA0228) (abinScience Cat# HT245013, RRID:AB_3729762)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Protein arginine N-methyltransferase 5 (SKB1/PRMT5) is a ~72 kDa protein. Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties. May methylate the N-terminal region of MBD2. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro.

1. Pollack, BP. et al. (1999) The Journal of biological chemistry 274, 31531-42. PMID: 10531356

2. Rho, J. et al. (2001) The Journal of biological chemistry 276, 11393-401. PMID: 11152681

3. Meister, G. et al. (2001) Current biology : CB 11, 1990-4. PMID: 11747828

4. Meister, G. et al. (2002) The EMBO journal 21, 5853-63. PMID: 12411503

5. Jiang, W. et al. (2005) Biochemical and biophysical research communications 329, 522-30. PMID: 15737618

6. Gonsalvez, GB. et al. (2007) The Journal of cell biology 178, 733-40. PMID: 17709427

7. Ren, J. et al. (2010) The Journal of biological chemistry 285, 12695-705. PMID: 20159986

8. Guo, S. et al. (2010) The Journal of biological chemistry 285, 35133-41. PMID: 20810653

9. Guderian, G. et al. (2011) The Journal of biological chemistry 286, 1976-86. PMID: 21081503

10. Hsu, JM. et al. (2011) Nature cell biology 13, 174-81. PMID: 21258366

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SKB1/PRMT5 IP Antibody (SAA0228)

Reférences

Fiche technique

Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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