ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
21.71 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
PRMT5, SKB1 homolog, 72 kDa ICln-binding protein, Jak-binding protein 1, Histone-arginine N-methyltransferase PRMT5, SKB1Hs, JBP1, Shk1 kinase-binding protein 1 homolog, SKB1, HRMT1L5, IBP72, Protein arginine N-methyltransferase 5
Background
Protein arginine N-methyltransferase 5 (SKB1/PRMT5) is a ~72 kDa protein. Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties. May methylate the N-terminal region of MBD2. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro.
1. Pollack, BP. et al. (1999) The Journal of biological chemistry 274, 31531-42. PMID: 10531356
2. Rho, J. et al. (2001) The Journal of biological chemistry 276, 11393-401. PMID: 11152681
3. Meister, G. et al. (2001) Current biology : CB 11, 1990-4. PMID: 11747828
4. Meister, G. et al. (2002) The EMBO journal 21, 5853-63. PMID: 12411503
5. Jiang, W. et al. (2005) Biochemical and biophysical research communications 329, 522-30. PMID: 15737618
6. Gonsalvez, GB. et al. (2007) The Journal of cell biology 178, 733-40. PMID: 17709427
7. Ren, J. et al. (2010) The Journal of biological chemistry 285, 12695-705. PMID: 20159986
8. Guo, S. et al. (2010) The Journal of biological chemistry 285, 35133-41. PMID: 20810653
9. Guderian, G. et al. (2011) The Journal of biological chemistry 286, 1976-86. PMID: 21081503
10. Hsu, JM. et al. (2011) Nature cell biology 13, 174-81. PMID: 21258366
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human PRMT5 Protein, N-His
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