Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228) (HT245013)

Name: Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228)
Brand: abinScience
SKU: HT245013
Price: 191 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT245013

Species reactivity

Human, Mouse

Applications

ELISA, IF, IP

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG1

Clone ID

SAA0228

Target

PRMT5, SKB1 homolog, 72 kDa ICln-binding protein, Jak-binding protein 1, Histone-arginine N-methyltransferase PRMT5, SKB1Hs, JBP1, Shk1 kinase-binding protein 1 homolog, SKB1, HRMT1L5, IBP72, Protein arginine N-methyltransferase 5

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O14744

RRID

Anti-Human SKB1/PRMT5 IP Antibody (SAA0228) (abinScience Cat# HT245013, RRID:AB_3729762)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protein arginine N-methyltransferase 5 (SKB1/PRMT5) is a ~72 kDa protein. Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties. May methylate the N-terminal region of MBD2. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro.

1. Pollack, BP. et al. (1999) The Journal of biological chemistry 274, 31531-42. PMID: 10531356

2. Rho, J. et al. (2001) The Journal of biological chemistry 276, 11393-401. PMID: 11152681

3. Meister, G. et al. (2001) Current biology : CB 11, 1990-4. PMID: 11747828

4. Meister, G. et al. (2002) The EMBO journal 21, 5853-63. PMID: 12411503

5. Jiang, W. et al. (2005) Biochemical and biophysical research communications 329, 522-30. PMID: 15737618

6. Gonsalvez, GB. et al. (2007) The Journal of cell biology 178, 733-40. PMID: 17709427

7. Ren, J. et al. (2010) The Journal of biological chemistry 285, 12695-705. PMID: 20159986

8. Guo, S. et al. (2010) The Journal of biological chemistry 285, 35133-41. PMID: 20810653

9. Guderian, G. et al. (2011) The Journal of biological chemistry 286, 1976-86. PMID: 21081503

10. Hsu, JM. et al. (2011) Nature cell biology 13, 174-81. PMID: 21258366

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SKB1/PRMT5 IP Antibody (SAA0228)

References

Datasheet

Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SKB1/PRMT5 IP Recombinant Antibody (SAA0228) (HT245013)

Overview

Images

References

Datasheet

SDS

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