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| カタログ番号 | HP320013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | ELISA, FCM, IF, IP | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG1 | ||||
| クローンID | SAA0230 | ||||
| ターゲット | Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, SMYD3, ZMYND1, Histone-lysine N-methyltransferase SMYD3, ZNFN3A1 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | Q9H7B4 | ||||
| RRID | Anti-Human SMYD3 IP Antibody (SAA0230) (abinScience Cat# HP320013, RRID:AB_3729764) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Histone-lysine N-methyltransferase SMYD3 is a ~49 kDa protein. Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. 1. Hamamoto, R. et al. (2004) Nature cell biology 6, 731-40. PMID: 15235609 2. Van Aller, GS. et al. (2012) Epigenetics 7, 340-3. PMID: 22419068 | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
Flow-cytometry using anti-human SMYD3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human SMYD3 antibody monoclonal antibody (Catalog # HP320013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human SMYD3 IP Antibody (SAA0230).
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