Anti-Human SPOP Polyclonal Antibody (HT175014)

Name: Anti-Human SPOP Polyclonal Antibody
Brand: abinScience
SKU: HT175014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HT175014

Description

Anti-Human SPOP Polyclonal Antibody (HT175014) is a rabbit polyclonal antibody detecting SPOP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SPOP (Ser21-Thr175).

Cible

Roadkill homolog 1, HIB homolog 1, SPOP, Speckle-type POZ protein

Purification

Purified by antigen affinity column.

Numéro d'accès

O43791

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Speckle-type POZ protein (SPOP) is a ~42 kDa protein. Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

1. Nabais Sá, MJ. et al. (2020) American journal of human genetics 106, 405-411. PMID: 32109420

2. Sanada, S. et al. (2023) Biochemical and biophysical research communications 651, 30-38. PMID: 36791496

3. Schwartz, I. et al. (2023) eLife 12. PMID: 37622993

4. Zang, L. et al. (2023) Journal of virology 97, e0078623. PMID: 37796126

5. Pi, Y. et al. (2023) Journal of medical virology 95, e29254. PMID: 38018242

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human SPOP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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