Human SPOP Recombinant Protein (N-His) (HT175012)

Name: Human SPOP Recombinant Protein (N-His)
Brand: abinScience
SKU: HT175012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT175012
Description	Recombinant Human SPOP Protein, N-His (HT175012) expressed in E. coli, spanning Ser21-Thr175. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O43791
Protein length	Ser21-Thr175
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.20 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Roadkill homolog 1, HIB homolog 1, SPOP, Speckle-type POZ protein
Background	Speckle-type POZ protein (SPOP) is a ~42 kDa protein. Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. 1. Nabais Sá, MJ. et al. (2020) American journal of human genetics 106, 405-411. PMID: 32109420 2. Sanada, S. et al. (2023) Biochemical and biophysical research communications 651, 30-38. PMID: 36791496 3. Schwartz, I. et al. (2023) eLife 12. PMID: 37622993 4. Zang, L. et al. (2023) Journal of virology 97, e0078623. PMID: 37796126 5. Pi, Y. et al. (2023) Journal of medical virology 95, e29254. PMID: 38018242
Note	For research use only.

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SDS-PAGE for Recombinant Human SPOP Protein, N-His

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Datasheet

Human SPOP Recombinant Protein (N-His).pdf

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Human SPOP Recombinant Protein (N-His) (HT175012)

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