Anti-Human SPOP Polyclonal Antibody (HT175014)

Name: Anti-Human SPOP Polyclonal Antibody
Brand: abinScience
SKU: HT175014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HT175014

Description

Anti-Human SPOP Polyclonal Antibody (HT175014) is a rabbit polyclonal antibody detecting SPOP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SPOP (Ser21-Thr175).

Target

Roadkill homolog 1, HIB homolog 1, SPOP, Speckle-type POZ protein

Purification

Purified by antigen affinity column.

Accession

O43791

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Speckle-type POZ protein (SPOP) is a ~42 kDa protein. Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

1. Nabais Sá, MJ. et al. (2020) American journal of human genetics 106, 405-411. PMID: 32109420

2. Sanada, S. et al. (2023) Biochemical and biophysical research communications 651, 30-38. PMID: 36791496

3. Schwartz, I. et al. (2023) eLife 12. PMID: 37622993

4. Zang, L. et al. (2023) Journal of virology 97, e0078623. PMID: 37796126

5. Pi, Y. et al. (2023) Journal of medical virology 95, e29254. PMID: 38018242

Note

For research use only.

Images

References

Datasheet

Anti-Human SPOP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SPOP Polyclonal Antibody (HT175014)

Overview

Images

References

Datasheet

SDS

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