Anti-Human SPOP Polyclonal Antibody (HT175014)

Name: Anti-Human SPOP Polyclonal Antibody
Brand: abinScience
SKU: HT175014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HT175014

Описание

Anti-Human SPOP Polyclonal Antibody (HT175014) is a rabbit polyclonal antibody detecting SPOP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human SPOP (Ser21-Thr175).

Цель

Roadkill homolog 1, HIB homolog 1, SPOP, Speckle-type POZ protein

Очистка

Purified by antigen affinity column.

Номер доступа

O43791

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Speckle-type POZ protein (SPOP) is a ~42 kDa protein. Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

1. Nabais Sá, MJ. et al. (2020) American journal of human genetics 106, 405-411. PMID: 32109420

2. Sanada, S. et al. (2023) Biochemical and biophysical research communications 651, 30-38. PMID: 36791496

3. Schwartz, I. et al. (2023) eLife 12. PMID: 37622993

4. Zang, L. et al. (2023) Journal of virology 97, e0078623. PMID: 37796126

5. Pi, Y. et al. (2023) Journal of medical virology 95, e29254. PMID: 38018242

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human SPOP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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