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Anti-Human SULT1E1 Polyclonal Antibody (HW729014)

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Anti-Human SULT1E1 Polyclonal Antibody
Anti-Human SULT1E1 Polyclonal Antibody
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Vista general
Número de catálogoHW729014
Descripción
Anti-Human SULT1E1 Polyclonal Antibody (HW729014) is a rabbit polyclonal antibody detecting SULT1E1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human SULT1E1 (Met1-Ile294 ).
Objetivo ST1E1, STE, EST-1, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring, Estrogen sulfotransferase
Purificación Purified by antigen affinity column.
Número de acceso P49888
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Sulfotransferase 1E1 (SULT1E1) is a ~35 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine. May play a role in gut microbiota-host metabolic interaction.

1. Hempel, N. et al. (2000) Biochemical and biophysical research communications 276, 224-30. PMID: 11006110
2. Pedersen, LC. et al. (2002) The Journal of biological chemistry 277, 17928-32. PMID: 11884392
3. Falany, CN. et al. (1995) The Journal of steroid biochemistry and molecular biology 52, 529-39. PMID: 7779757
4. Cook, IT. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 2069-78. PMID: 19589875
Nota For research use only.
Imágenes
  • Anti-Human SULT1E1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SULT1E1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse liver lysate
    Lane 4: Mouse kidney lysate
    Lane 5: Mouse small intestine lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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