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Anti-Human SULT1E1 Recombinant Antibody (SAA0759) (HW729023)

Anti-Human SULT1E1 Recombinant Antibody (SAA0759)
Anti-Human SULT1E1 Recombinant Antibody (SAA0759)
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  • 100ug
  • 1mg
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Overview
Catalog No.HW729023
Description
Anti-Human SULT1E1 Recombinant Antibody (SAA0759) (HW729023) is a rabbit monoclonal antibody detecting SULT1E1 in ELISA, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, WB
Host speciesRabbit
ClonalityMonoclonal
IsotypeIgG
Clone IDSAA0759
Target ST1E1, STE, EST-1, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring, Estrogen sulfotransferase
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P49888
RRID

Anti-Human SULT1E1 Antibody (SAA0759) (abinScience Cat# HW729023, RRID:AB_3726305)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Sulfotransferase 1E1 (SULT1E1) is a ~35 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine. May play a role in gut microbiota-host metabolic interaction.

1. Hempel, N. et al. (2000) Biochemical and biophysical research communications 276, 224-30. PMID: 11006110
2. Pedersen, LC. et al. (2002) The Journal of biological chemistry 277, 17928-32. PMID: 11884392
3. Falany, CN. et al. (1995) The Journal of steroid biochemistry and molecular biology 52, 529-39. PMID: 7779757
4. Cook, IT. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 2069-78. PMID: 19589875
Note For research use only.
Images
  • Anti-Human SULT1E1 Recombinant Antibody (SAA0759)

    SDS-PAGE

    SDS-PAGE for Anti-Human SULT1E1 Antibody (SAA0759)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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Working Solution
C₂ (Working Conc.)
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