

| Catalog No. | HW729033 |
|---|---|
| Species reactivity | Human |
| Applications | IF |
| Host species | Rabbit |
| Isotype | IgG |
| Clone ID | SRR0173 |
| Clonality | Monoclonal |
| Tested applications | IF |
| Target | EC:2.8.2.4, EST-1, Estrogen sulfotransferase, ST1E1, STE, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P49888 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Note | For research use only |

HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting SULT1E1 (HW729033) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of SULT1E1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).




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