Anti-Human SULT1E1 Recombinant Antibody (SRR0173) (HW729033)

Name: Anti-Human SULT1E1 Recombinant Antibody (SRR0173)
Brand: abinScience
SKU: HW729033
Price: 191 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	HW729033
Description	Anti-Human SULT1E1 Recombinant Antibody (SRR0173) (HW729033) is a rabbit monoclonal antibody detecting SULT1E1 in IF. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivity	Human
Applications	IF
Host species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone ID	SRR0173
Tested applications	IF
Target	EC:2.8.2.4, EST-1, Estrogen sulfotransferase, ST1E1, STE, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P49888
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Sulfotransferase 1E1 (SULT1E1) is a ~35 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine. May play a role in gut microbiota-host metabolic interaction. 1. Hempel, N. et al. (2000) Biochemical and biophysical research communications 276, 224-30. PMID: 11006110 2. Pedersen, LC. et al. (2002) The Journal of biological chemistry 277, 17928-32. PMID: 11884392 3. Falany, CN. et al. (1995) The Journal of steroid biochemistry and molecular biology 52, 529-39. PMID: 7779757 4. Cook, IT. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 2069-78. PMID: 19589875
Note	For research use only.

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Immunofluorescence

HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting SULT1E1 (HW729033) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of SULT1E1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet

Anti-Human SULT1E1 Recombinant Antibody (SRR0173).pdf

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Formula

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Recombinant Proteins

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Anti-Human SULT1E1 Recombinant Antibody (SRR0173) (HW729033)

Overview

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References

Datasheet

SDS