Anti-Human TIGIT Recombinant Antibody (SAA0154), PE (HS739227)

Name: Anti-Human TIGIT Recombinant Antibody (SAA0154), PE
Brand: abinScience
SKU: HS739227
Price: 290 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HS739227
Reactivité des espèces	Human
Applications	FCM
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	SAA0154
Conjugaison	PE
Applications testées	FCM
Cible	T-cell immunoreceptor with Ig and ITIM domains, TIGIT, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, VSIG9, VSTM3
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Produits associés	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
Numéro d'accès	Q495A1
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a ~26 kDa protein. Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. 1. Yu, X. et al. (2009) Nature immunology 10, 48-57. PMID: 19011627 2. Liu, S. et al. (2013) Cell death and differentiation 20, 456-64. PMID: 23154388 3. Li, M. et al. (2014) The Journal of biological chemistry 289, 17647-57. PMID: 24817116 4. Reches, A. et al. (2020) Journal for immunotherapy of cancer 8. PMID: 32503945
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow cytometry

Flow-cytometry PE using anti-human TIGIT antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human TIGIT monoclonal antibody (Catalog HS739227) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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